0100-0456 was raised against Dehydroepiandrosterone (DHEA) but demonstrates greater reactivity with Dehydroepiandrosterone Sulfate (DHEAS):
DHEAS
100%
DHEA
17%
Progesterone
0.008%
Testosterone
0.004%
DHEA is the most abundant hormone in the human body and a precursor of all the sex hormones. It is synthesized principally in the adrenal glands and gonads from cholesterol, through conversion to pregnenolone and 17a-Hydroxypregnenolone, by the action of P450 enzymes.
DHEA is mainly found in serum as the sulfated form Dehydroepiandrosterone Sulfate (DHEAS), produced through a reversible reaction catalysed by sulfotransferase in the adrenal glands, liver and small intestine.
Target Species
Broad
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
DHEA-3-HS-BSA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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