0100-0420 detects glycogen phosphorylase BB (GPBB), a regulatory enzyme which catalyses the rate-determining step in glycogen degradation. In unstable angina, rapid rises in plasma concentrations of GPBB have been observed. Mammalian glycogen phosphorylase is found as three major isozymes including muscle (GPMM), liver (GPLL), and brain/heart (GPBB), which can be distinguished by their structural and functional properties as well as by the tissues in which they are expressed.
0100-0420 also reacts with GPMM.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human Glycogen Phosphorylase BB.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp 2/0 myeloma cell line.
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