3H11 reacts with an epitope dependent on the last 84 amino acids of rat NF-M (145 kD). Neurofilament proteins consist of the light NF-L, medium NF-M and heavy NF-H subunits.Most neurons appear to express these proteins but their ratios and phosphorylation states are variable.
Clone 3H11 has also been determined to bind to a C- terminal epitope of bovine NF-M SEE IMAGE. from: Shawet al. 2004
Target Species
Rat
Species Cross-Reactivity
Target Species
Cross Reactivity
Birds
Yes
Bovine
Yes
Mammals
Yes
Product Form
Ig fraction - liquid
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fusion protein RM:677-845 containing the last 169 amino acids of rat neurofilament (145 kD). Purified from E. coli inclusion bodies by Ion exchange chromatography. Corresponds to the rat NF-M KE segment (lysine glutamate) (ref.1)
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
1/50 - 1/1000
Western Blotting
1/1K - 1/2K
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
