0100-0190 is specific for Neuronal Calcium Sensor 1, also known as Frequenin or NCS1, part of a large family of calcium-dependent proteins that act as molecular switches.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
0100-0190 detects a band of approximately 29kDa in rat brain homogenates under reducing conditions.
Target Species
Rat
Species Cross-Reactivity
Target Species
Cross Reactivity
Chicken
Expected from Sequence
Human
Expected from Sequence
Mouse
Expected from Sequence
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the C terminal region of the native molecule.
Approx. Protein Concentrations
IgG concentration 5.0mg/ml
Buffer Solution
Phosphate buffered saline
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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