Secretin is a polypeptide hormone produced by the S-cells of the duodenum in response to a low pH. This stimultes bicarbonate secretion from various sources that acts as a buffer in order to maintain a constant pH.
This antibody exhibits the following reactivities:
Secretin (human)
100%
Secretin (porcine)
140%
Neuromedin N
0.001%
Neuromedin U-25
<=0.001%
VIP (porcine)
<=0.001%
Substance P
<=0.001%
Somatostatin
<=0.001%
Target Species
Human
Species Cross-Reactivity
Target Species
Cross Reactivity
Pig
Yes
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilisers
0.09%
Sodium Azide (NaN3)
Immunogen
Synthetic peptide HSDGTFTSELSRLREGARLQRLLQGLV corresponding to part of the native molecule.
Approx. Protein Concentrations
5 mg/ml
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Paraffin(1)
1/50 - 1/100
(1)
This product requires antigen retrieval using heat treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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