0100-0119 recognises Thyroxine, also known as T4, the major hormone secreted by the follicular cells of the thyroid gland. Thyroxine's main role is to regulate metabolic processes in the body such as heart rate, along with Triiodothyronine (T3). T4 is involved in thyroid disorders, such as hyperthyroidism and hypothyroidism.
0100-0119 shows low levels of reactivity with biologically inactive rT3 (approximately 10%) and minimal reactivity with T3 (approximately 1.5%).
Target Species
Broad
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic thyroxine conjugated to Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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