0100-0083 is specific for calnexin, a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum.
Target Species
Dog
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Guinea Pig
Yes
Hamster
Yes
Human
Yes
Monkey
Yes
Mouse
Yes
Rabbit
Yes
Rat
Yes
Sheep
Yes
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Synthetic peptide, AEEDEILNRSPRNRKPRRE, conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
1 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
0100-0083 detects a band of approximately 90 kDa in western blotting using heat-shocked HeLa cell lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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