0100-0082 is specific for ankyrin G and reacts with a region within the spectrin binding domain. In Western blotting it detects the 190 kDa isoform.
Ankyrins are a family of spectrin-binding protein that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel and the Na/K ATPase. 3 forms of ankyrin G exists, 190 kDa, 270 kDa and 480 kDa, that are localized in the axonal intial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched.
Target Species
Human
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Yes
Mouse
Yes
Rat
Yes
Product Form
Purified IgG - liquid
Preservative Stabilisers
0.09%
Sodium Azide (NaN3)
0.2%
Gelatin
Immunogen
A synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin G
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunofluorescence
1-2 µg/ml
Immunohistology - Frozen
1-2 µg/ml
Immunohistology - Paraffin
Western Blotting
2-5 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3X63/Ag 8.653 mouse myeloma cell line
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