0100-0075 is specific for the dopamine transporter, N-terminus. It does not cross react with serotonin and norepinephrine transporters.
Target Species
Human
Species Cross-Reactivity
Target Species
Cross Reactivity
Monkey
Yes
Mouse
Yes
Rat
Yes
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
None present
Immunogen
N-terminus of human dopamine transporter fused to Glutathione S-transferase.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/1000 - 1/10 000
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
0100-0075 recognises a diffuse band at approximatley 70-85 kDa on extracts from human caudate, putamen and nucleus accumbens.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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