0100-0074 recognises an extracellular region close to the C terminal end of the alpha subunit of human cranin, also known as dystroglycan. Cranin is expressed in brain and many other tissues, it binds laminin with high affinity in a calcium dependent manner. It appears to be important in maintaining normal muscle integrity. Loss of cranin from the muscle surface may be one of the primary events leading to muscle injury in congenital muscular dystrophies, it may also play a role in the cognitive deficits often seen in such conditions.
Target Species
Human
Species Cross-Reactivity
Target Species
Cross Reactivity
Chicken
Yes
Mouse
Yes
Rat
Yes
Sheep
Yes
Xenopus
Yes
Product Form
Ascites - liquid
Preservative Stabilisers
None present
Immunogen
A synthetic peptide corresponding to amino acids 572-604 of human cranin (dystroglycan).
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
1/1 - 1/50
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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