0093-5059 recognises human activating transcription factor 2 (ATF2), a nuclear protein that promotes transcription in the presence of cyclic AMP.
This antibody does not react with ATF1 or ATF3.
Target Species
Human
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Horse
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
An Escherichia coli-derived GST fusion protein corresponding to the N-terminal (amino acids 95 to 211) of human ATF2.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
1 - 10ug/ml
Western Blotting
1 - 10ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
This antibody recognises a band at approximately 65kDa in HeLa nuclear extracts.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.