0070-2304 reacts with actin, the major constitutent of the thin filaments in muscle and of the microfilaments in non-muscle cells. It is present in all eukaryotic cells and its organization may vary from one cell to another depending on it's origin and physiological state. Actin exists a monomeric form (G-actin) or in a filamentous form (F-actin).
In immunofluorescence, this product stains the stress fibres of cultured fibroblasts from chicken, mouse, rat and human. It also stains the I-bands of stretched skeletal muscle.
Species Cross-Reactivity
Target Species
Cross Reactivity
Chicken
Yes
Human
Yes
Mouse
Yes
Rat
Yes
Product Form
Serum - liquid
Preservative Stabilisers
0.09%
Sodium Azide (NaN3)
Immunogen
Actin isolated from chicken back muscle.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunofluorescence
1/40
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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