Description: eBioscience Intracellular (IC) Fixation Buffer, used in conjunction with eBioscience Permeabilization Buffer (Cat. No. 00-8333), can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens. These solutions have been specially formulated for reducing non-specific staining by fluorochrome-labelled antibodies and increasing signal to noise ratios.