Description: The Foxp3 Fixation/Permeabilization Concentrate and Diluent solutions have been formulated and optimized for Foxp3 staining with the FJK-16s, NRRF-30, PCH101, 236A/E7 and eBio7979 monoclonal antibodies. It has also been shown to work for other transcription factors suchs as Nanog, Tbet, Gata-3, Ror-gamma as well as cytokines.
Components
eBioscience Fixation/Permeabilization Concentrate: 30 ml. Store at 4°C. Avoid agitation. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Fixation/Permeabilization Concentrate with 3 parts Fixation/Permeabilization Diluent. Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves. eBioscience Fixation/Permeabilization Diluent: 100 ml. Store at 4°C. The diluent is intented to be used in combination with the Fixation/Permeabilization Concentrate.
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